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验证数据展示

  • WB analysis of rat brain using 66709-1-Ig

    WB analysis of rat brain using 66709-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66709-1-Ig

    IHC staining of mouse brain using 66709-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAG Monoclonal antibody

MAG Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66709-1-Ig

产品说明书

CloneNo. 3H9G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66709-1-Ig targets MAG in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MAG fusion protein Ag6065 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myelin associated glycoprotein
别名 GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A
计算分子量 69 kDa
观测分子量 100 kDa
GenBank蛋白编号BC053347
基因名称 MAG
Gene ID (NCBI) 4099
RRIDAB_2882061
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 66709-1-IgDownload protocol
IHC protocol for MAG antibody 66709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols