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MAGEA4 Recombinant antibody

MAGEA4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241558G7

Cat no : 84248-4-RR

Print datasheet

Synonyms

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

验证数据展示

  • WB analysis using 84248-4-RR

    WB analysis using 84248-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84248-4-RR

    FC experiment of A431 using 84248-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84248-4-RR

    Affinity and Kinetic Characterization of 84248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

经过测试的应用

Positive WB detected inA431 cells, human testis tissue, U2OS cells, A375 cells
Positive FC (Intra) detected inA431 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84248-4-RR targets MAGEA4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MAGEA4 fusion protein Ag3193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name melanoma antigen family A, 4
Synonyms MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 84248-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols