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验证数据展示

  • WB analysis using 84248-4-RR

    WB analysis using 84248-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84248-4-RR

    FC experiment of A431 using 84248-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-4-RR, Clone:241558G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84248-4-RR

    Affinity and Kinetic Characterization of 84248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

MAGEA4 Recombinant antibody

MAGEA4 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84248-4-RR

产品说明书

CloneNo. 241558G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, human testis tissue, U2OS cells, A375 cells
Positive FC (Intra) detected inA431 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84248-4-RR targets MAGEA4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MAGEA4 fusion protein Ag3193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4
计算分子量 317 aa, 35 kDa
观测分子量45 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43358
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 84248-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols