验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
产品信息
66225-1-Ig targets MALT1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MALT1 fusion protein Ag20632 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mucosa associated lymphoid tissue lymphoma translocation gene 1 |
| 别名 | 4D3F6, EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT |
| 计算分子量 | 824 aa, 92 kDa |
| 观测分子量 | 92-100 kDa |
| GenBank蛋白编号 | BC030143 |
| 基因名称 | MALT1 |
| Gene ID (NCBI) | 10892 |
| RRID | AB_2881616 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UDY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MALT1 antibody 66225-1-Ig | Download protocol |
| IHC protocol for MALT1 antibody 66225-1-Ig | Download protocol |
| IF protocol for MALT1 antibody 66225-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol The COP9 signalosome stabilized MALT1 promotes Non-Small Cell Lung Cancer progression through activation of NF-κB pathway | ||
Front Cell Dev Biol Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis. |
