MAML3 Polyclonal antibody
MAML3 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 23263-1-AP is MAML3 Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | MAML3 fusion protein Ag19788 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mastermind-like 3 (Drosophila) |
Synonyms | CAGH3, ERDA3, GDN, KIAA1816, MAM 2, Mam 3, MAM2, MAML3, mastermind like 3 (Drosophila), Mastermind like protein 3, TNRC3 |
Calculated Molecular Weight | 1133 aa, 122 kDa |
Observed Molecular Weight | 122 kDa |
GenBank Accession Number | BC137131 |
Gene Symbol | MAML3 |
Gene ID (NCBI) | 55534 |
RRID | AB_2879242 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96JK9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.
实验方案
Product Specific Protocols | |
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WB protocol for MAML3 antibody 23263-1-AP | Download protocol |
IF protocol for MAML3 antibody 23263-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |