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验证数据展示

  • WB analysis of HEK-293 using 23263-1-AP

    WB analysis of HEK-293 using 23263-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23263-1-AP (MAML3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 23263-1-AP

    IF Staining of HEK-293 using 23263-1-AP

    Immunofluorescent analysis of HEK-293 cells using 23263-1-AP (MAML3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAML3 Polyclonal antibody

MAML3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23263-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CAGH3, ERDA3, GDN, KIAA1816, MAM 2, Mam 3, MAM2, MAML3, mastermind like 3 (Drosophila), Mastermind like protein 3, TNRC3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23263-1-AP targets MAML3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MAML3 fusion protein Ag19788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mastermind-like 3 (Drosophila)
别名 CAGH3, ERDA3, GDN, KIAA1816, MAM 2, Mam 3, MAM2, MAML3, mastermind like 3 (Drosophila), Mastermind like protein 3, TNRC3
计算分子量 1133 aa, 122 kDa
观测分子量 122 kDa
GenBank蛋白编号BC137131
基因名称 MAML3
Gene ID (NCBI) 55534
RRIDAB_2879242
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JK9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.

实验方案

Product Specific Protocols
WB protocol for MAML3 antibody 23263-1-APDownload protocol
IF protocol for MAML3 antibody 23263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols