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验证数据展示

  • WB analysis using 68471-1-Ig

    WB analysis using 68471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 68471-1-Ig

    WB analysis of U2OS using 68471-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68471-1-Ig

    WB analysis of LNCaP using 68471-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68471-1-Ig (MAOA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 68471-1-Ig

    IHC staining of human pancreas cancer using 68471-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 68471-1-Ig

    IHC staining of human pancreas cancer using 68471-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68471-1-Ig (MAOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 68471-1-Ig

    FC experiment of HepG2 using 68471-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAOA Monoclonal antibody (68471-1-Ig, Clone:1A9E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MAOA Monoclonal antibody

MAOA Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 68471-1-Ig

产品说明书

CloneNo. 1A9E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

monoamine oxidase A, MAO-A, MAO A, EC:1.4.3.21, Amine oxidase [flavin-containing] A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, U2OS cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68471-1-Ig targets MAOA in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MAOA fusion protein Ag30028 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 monoamine oxidase A
别名 monoamine oxidase A, MAO-A, MAO A, EC:1.4.3.21, Amine oxidase [flavin-containing] A
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC008064
基因名称 MAOA
Gene ID (NCBI) 4128
RRIDAB_3085182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

实验方案

Product Specific Protocols
WB protocol for MAOA antibody 68471-1-IgDownload protocol
IHC protocol for MAOA antibody 68471-1-IgDownload protocol
FC protocol for MAOA antibody 68471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols