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验证数据展示

  • WB analysis of fetal human brain using 66107-1-Ig

    WB analysis of fetal human brain using 66107-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66107-1-Ig

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens).

  • IHC staining of human brain using 66107-1-Ig

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens).

  • WB analysis of human brain using 66107-1-Ig

    WB analysis of human brain using 66107-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66107-1-Ig

    WB analysis of HeLa using 66107-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66107-1-Ig

    WB analysis using 66107-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 66107-1-Ig

    IF Staining of mouse kidney using 66107-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAOB Monoclonal antibody

MAOB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66107-1-Ig

产品说明书

CloneNo. 2B12F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, human kidney tissue, HeLa cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66107-1-Ig targets MAOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 MAOB fusion protein Ag17918 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 monoamine oxidase B
别名 monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B
计算分子量 520 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC022494
基因名称 MAOB
Gene ID (NCBI) 4129
RRIDAB_2881506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27338
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).

实验方案

Product Specific Protocols
WB protocol for MAOB antibody 66107-1-IgDownload protocol
IHC protocol for MAOB antibody 66107-1-IgDownload protocol
IF protocol for MAOB antibody 66107-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols