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MAOB Monoclonal antibody

MAOB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B12F3

Cat no : 66107-1-Ig

Print datasheet

Synonyms

MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B

验证数据展示

  • WB analysis of fetal human brain using 66107-1-Ig

    WB analysis of fetal human brain using 66107-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66107-1-Ig

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens).

  • IHC staining of human brain using 66107-1-Ig

    IHC staining of human brain using 66107-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens).

  • IF Staining of mouse brain using 66107-1-Ig

    IF Staining of mouse brain using 66107-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 66107-1-Ig

    IF Staining of mouse brain using 66107-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:40 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of human brain using 66107-1-Ig

    WB analysis of human brain using 66107-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66107-1-Ig

    WB analysis of HeLa using 66107-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66107-1-Ig

    WB analysis using 66107-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, human kidney tissue, HeLa cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66107-1-Ig targets MAOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MAOB fusion protein Ag17918 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name monoamine oxidase B
Synonyms MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B
Calculated Molecular Weight 520 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC022494
Gene Symbol MAOB
Gene ID (NCBI) 4129
RRIDAB_2881506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27338
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).

实验方案

Product Specific Protocols
WB protocol for MAOB antibody 66107-1-IgDownload protocol
IHC protocol for MAOB antibody 66107-1-IgDownload protocol
IF protocol for MAOB antibody 66107-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols