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验证数据展示

  • WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    WB analysis of fetal human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    IHC staining of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66107-1-Ig (MAOB Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    WB analysis of human brain using 66107-1-Ig (same clone as 66107-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

    WB analysis of HeLa using 66107-1-Ig (same clone as 66107-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

    WB analysis using 66107-1-Ig (same clone as 66107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66107-1-Ig (MAOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 66107-1-Ig (same clone as 66107-1-PBS)

    IF Staining of mouse kidney using 66107-1-Ig (same clone as 66107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using MAOB antibody (66107-1-Ig, Clone: 2B12F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66107-1-PBS in a different storage buffer formulation.

MAOB Monoclonal antibody, PBS Only

MAOB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66107-1-PBS

产品说明书

CloneNo. 2B12F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66107-1-PBS targets MAOB in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 MAOB fusion protein Ag17918 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 monoamine oxidase B
别名 monoamine oxidase B, MAO-B, MAO B, EC:1.4.3.21, Amine oxidase [flavin-containing] B
计算分子量 520 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC022494
基因名称 MAOB
Gene ID (NCBI) 4129
RRIDAB_2881506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27338
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).