验证数据展示
经过测试的应用
| Positive IHC detected in | mouse cerebellum tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55062-1-AP targets MAP1A in IHC, ELISA applications and shows reactivity with mouse, rat, human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | mouse, rat, human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1A |
| 别名 | LC2, MAP1A, MAP1A protein, MAP1L, MTAP1A |
| 计算分子量 | 305 kDa |
| GenBank蛋白编号 | NM_002373 |
| 基因名称 | MAP1A |
| Gene ID (NCBI) | 4130 |
| RRID | AB_2918355 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78559 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The assembly of microtubules, an essential step in neurogenesis, is modulated by the family of microtubule-associated proteins (MAPs). These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B , and MAP2 , and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1A is the largest brain MAP and expressed prominently in dendrites and to a lesser extent in cell bodies and axons . This protein is involved in differentiation of axons and dendrites as well as maintenance of the microtubule cytoskeleton in mature neurons.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP1A antibody 55062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
