验证数据展示
经过测试的应用
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67423-1-Ig targets MAP1B in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | rat |
| Immunogen | MAP1B fusion protein Ag17709 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | microtubule-associated protein 1B |
| Synonyms | DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5 |
| Calculated Molecular Weight | 2468 aa, 271 kDa |
| GenBank Accession Number | BC141853 |
| Gene Symbol | MAP1B |
| Gene ID (NCBI) | 4131 |
| RRID | AB_2918490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P46821 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| IF protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| FC protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
