• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAP4 Monoclonal antibody, PBS Only

MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 68271-1-PBS

Print datasheet

Synonyms

Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6

验证数据展示

  • WB analysis using 68271-1-Ig (same clone as 68271-1-PBS)

    WB analysis using 68271-1-Ig (same clone as 68271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68271-1-Ig (same clone as 68271-1-PBS)

    IF Staining of A431 using 68271-1-Ig (same clone as 68271-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAP4 antibody (68271-1-Ig, Clone: 1C1A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

产品信息

68271-1-PBS targets MAP4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MAP4 fusion protein Ag1741 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 4
Synonyms Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6
Calculated Molecular Weight 121 kDa
Observed Molecular Weight210-240 kDa
GenBank Accession NumberBC012794
Gene Symbol MAP4
Gene ID (NCBI) 4134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27816
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)