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验证数据展示

  • WB analysis using 68271-1-Ig

    WB analysis using 68271-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68271-1-Ig

    IHC staining of human lung cancer using 68271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 68271-1-Ig

    IHC staining of human lung cancer using 68271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68271-1-Ig

    IF Staining of A431 using 68271-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAP4 antibody (68271-1-Ig, Clone: 1C1A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MAP4 Monoclonal antibody

MAP4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68271-1-Ig

产品说明书

CloneNo. 1C1A6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68271-1-Ig targets MAP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MAP4 fusion protein Ag1741 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 microtubule-associated protein 4
别名 Microtubule-associated protein 4, MAP-4, MAP 4, 1C1A6
计算分子量 121 kDa
观测分子量210-240 kDa
GenBank蛋白编号BC012794
基因名称 MAP4
Gene ID (NCBI) 4134
RRIDAB_2935354
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27816
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

实验方案

Product Specific Protocols
WB protocol for MAP4 antibody 68271-1-IgDownload protocol
IHC protocol for MAP4 antibody 68271-1-IgDownload protocol
IF protocol for MAP4 antibody 68271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols