• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated MAP4 Monoclonal antibody

MAP4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1A6

Cat no : CL488-68271

Print datasheet

Synonyms

验证数据展示

  • IF Staining of A431 using CL488-68271

    IF Staining of A431 using CL488-68271

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MAP4 antibody (CL488-68271, Clone: 1C1A6 ) at dilution of 1:200.

  • IF Staining of A431 using CL488-68271

    IF Staining of A431 using CL488-68271

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MAP4 antibody (CL488-68271, Clone: 1C1A6 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68271-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68271 targets MAP4 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MAP4 fusion protein Ag1741 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 4
Synonyms
Calculated Molecular Weight 121 kDa
Observed Molecular Weight210-240 kDa
GenBank Accession NumberBC012794
Gene Symbol MAP4
Gene ID (NCBI) 4134
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27816
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAP4 antibody CL488-68271Download protocol
Standard Protocols
Click here to view our Standard Protocols