验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 68271-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68271 targets MAP4 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | MAP4 fusion protein Ag1741 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 4 |
| 别名 | |
| 计算分子量 | 121 kDa |
| 观测分子量 | 210-240 kDa |
| GenBank蛋白编号 | BC012794 |
| 基因名称 | MAP4 |
| Gene ID (NCBI) | 4134 |
| RRID | AB_3672985 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P27816 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MAP4 antibody CL488-68271 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |