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MAP6/STOP Polyclonal antibody

MAP6/STOP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25717-1-AP

Print datasheet

Synonyms

KIAA1878, MAP 6, MAP6, MAP6/STOP, MTAP6, N STOP, Stable tubule only polypeptide, STOP

验证数据展示

  • WB analysis using 25717-1-AP

    WB analysis using 25717-1-AP

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 25717-1-AP (MAP6/STOP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 25717-1-AP

    IHC staining of mouse cerebellum using 25717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 25717-1-AP

    IHC staining of mouse cerebellum using 25717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25717-1-AP (MAP6/STOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 25717-1-AP

    IF Staining of PC-12 using 25717-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using MAP6/STOP antibody (25717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse retina tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25717-1-AP targets MAP6/STOP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MAP6/STOP fusion protein Ag22471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein 6
Synonyms KIAA1878, MAP 6, MAP6, MAP6/STOP, MTAP6, N STOP, Stable tubule only polypeptide, STOP
Calculated Molecular Weight 813 aa, 87 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC139780
Gene Symbol MAP6
Gene ID (NCBI) 4135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JE9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP6, also named STOP protein, has been isolated from brain preparations but is also present in other tissues such as the skeletal muscles and heart. MAP6 is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Different isoforms of STOP protein arise from alternative mRNA splicing and transcription initiation sites using a single gene. The neuronal MAP6 isoforms N-STOP (120 kDa) and E-STOP (80 kDa), the astrocyte MAP6 isoform A-STOP (60 KDa), and a fibroblastic MAP6 isoform F-STOP (48 kDa) have been identified.

实验方案

Product Specific Protocols
WB protocol for MAP6/STOP antibody 25717-1-APDownload protocol
IHC protocol for MAP6/STOP antibody 25717-1-APDownload protocol
IF protocol for MAP6/STOP antibody 25717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols