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验证数据展示

  • WB analysis using 20788-1-AP

    WB analysis using 20788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20788-1-AP (MAP7D1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20788-1-AP

    IP experiment of HEK-293 using 20788-1-AP

    IP result of anti-MAP7D1 (IP:20788-1-AP, 4ug; Detection:20788-1-AP 1:30000) with HEK-293 cells lysate 1470 ug.

  • IHC staining of mouse heart using 20788-1-AP

    IHC staining of mouse heart using 20788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 20788-1-AP

    IHC staining of mouse skeletal muscle using 20788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 20788-1-AP

    IF Staining of U-251 using 20788-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

MAP7D1 Polyclonal antibody

MAP7D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20788-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Proline/arginine-rich coiled-coil domain-containing protein 1, PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MDA-MB-231 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20788-1-AP targets MAP7D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 MAP7D1 fusion protein Ag14385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAP7 domain containing 1
别名 Proline/arginine-rich coiled-coil domain-containing protein 1, PP2464, PARCC1, MAP7 domain-containing protein 1, KIAA1187
计算分子量 841 aa, 93 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC003083
基因名称 MAP7D1
Gene ID (NCBI) 55700
RRIDAB_3669347
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KQU3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).

实验方案

Product Specific Protocols
WB protocol for MAP7D1 antibody 20788-1-APDownload protocol
IHC protocol for MAP7D1 antibody 20788-1-APDownload protocol
IF protocol for MAP7D1 antibody 20788-1-APDownload protocol
IP protocol for MAP7D1 antibody 20788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols