MAPKAPK2 Polyclonal antibody
MAPKAPK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue |
Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 10 publications below |
IHC | See 2 publications below |
产品信息
13949-1-AP targets MAPKAPK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | MAPKAPK2 fusion protein Ag5060 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase-activated protein kinase 2 |
Synonyms | MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2 |
Calculated Molecular Weight | 400 aa, 46 kDa |
Observed Molecular Weight | 47-50 kDa |
GenBank Accession Number | BC036060 |
Gene Symbol | MAPKAPK2 |
Gene ID (NCBI) | 9261 |
RRID | AB_2877995 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P49137 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
IHC protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
IF protocol for MAPKAPK2 antibody 13949-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Res Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2. | ||
Food Funct L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice. | ||
Toxicol Appl Pharmacol p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease. | ||
Int J Mol Med Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway. | ||
Brain Res Bull The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain. | ||
Cell Rep MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. |