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  • Featured Product
  • KD/KO Validated

MAPRE2 Polyclonal antibody

MAPRE2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10364-1-AP

Print datasheet

Synonyms

RP1, Microtubule-associated protein RP/EB family member 2, End-binding protein 2, EB2, APC-binding protein EB2

验证数据展示

  • WB analysis of K-562 using 10364-1-AP

    WB analysis of K-562 using 10364-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cells using 10364-1-AP

    IF Staining of mouse cells using 10364-1-AP

    Mouse keratinocyte showing MAPRE2 (#10364-1-AP, 1:100 dilution, with Alexa 594 labelled 2nd Ab) in red and alpha-tubulin (#66031-1-lg, 1:100 dilution, with Alexa 488 labelled 2nd Ab ) in green.

  • IHC staining of human stomach using 10364-1-AP

    IHC staining of human stomach using 10364-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10364-1-AP (MAPRE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10364-1-AP targets MAPRE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen MAPRE2 fusion protein Ag0416 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein, RP/EB family, member 2
Synonyms RP1, Microtubule-associated protein RP/EB family member 2, End-binding protein 2, EB2, APC-binding protein EB2
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC007318
Gene Symbol MAPRE2
Gene ID (NCBI) 10982
RRIDAB_2141649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RP1 protein shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family and binds specifically to the C-terminal region of the APC protein. RP1 also binds directly to tubulin, both in vitro and in vivo. The function of RP1 is currently unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. It may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

实验方案

Product Specific Protocols
WB protocol for MAPRE2 antibody 10364-1-APDownload protocol
IHC protocol for MAPRE2 antibody 10364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article
mouseWB

Exp Cell Res

MAPRE2 regulates the first meiotic progression in mouse oocytes.

Authors - Yuan-Yuan Li
  • KD Validated
View Article