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验证数据展示

  • WB analysis using 67408-1-Ig

    WB analysis using 67408-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67408-1-Ig (MAT1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67408-1-Ig

    IF Staining of HeLa using 67408-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT1A antibody (67408-1-Ig, Clone: 2F1C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MAT1A Monoclonal antibody

MAT1A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67408-1-Ig

产品说明书

CloneNo. 2F1C1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67408-1-Ig targets MAT1A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MAT1A fusion protein Ag3076 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methionine adenosyltransferase I, alpha
别名 AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1
计算分子量 395 aa, 44 kDa
观测分子量 49 kDa
GenBank蛋白编号BC018359
基因名称 MAT1A
Gene ID (NCBI) 4143
RRIDAB_2882649
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00266
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.

实验方案

Product Specific Protocols
WB protocol for MAT1A antibody 67408-1-IgDownload protocol
IF protocol for MAT1A antibody 67408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols