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CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody

MAT1A Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F1C1

Cat no : CL488-67408

Print datasheet

Synonyms

MAT I/III, MAT 1, MAT, EC:2.5.1.6, AMS1

验证数据展示

  • IF Staining of HeLa using CL488-67408

    IF Staining of HeLa using CL488-67408

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 MAT1A antibody (CL488-67408, Clone: 2F1C1 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67408

    FC experiment of HeLa using CL488-67408

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody (CL488-67408, Clone:2F1C1)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67408-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67408 targets MAT1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MAT1A fusion protein Ag3076 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name methionine adenosyltransferase I, alpha
Synonyms MAT I/III, MAT 1, MAT, EC:2.5.1.6, AMS1
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC018359
Gene Symbol MAT1A
Gene ID (NCBI) 4143
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00266
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
FC protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
Standard Protocols
Click here to view our Standard Protocols