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验证数据展示

  • IF Staining of HeLa using CL488-67408

    IF Staining of HeLa using CL488-67408

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 MAT1A antibody (CL488-67408, Clone: 2F1C1 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67408

    FC experiment of HeLa using CL488-67408

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody (CL488-67408, Clone:2F1C1)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody

MAT1A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67408

产品说明书

CloneNo. 2F1C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

MAT I/III, MAT 1, MAT, EC:2.5.1.6, AMS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67408-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67408 targets MAT1A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MAT1A fusion protein Ag3076 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 methionine adenosyltransferase I, alpha
别名 MAT I/III, MAT 1, MAT, EC:2.5.1.6, AMS1
计算分子量 395 aa, 44 kDa
观测分子量 49 kDa
GenBank蛋白编号BC018359
基因名称 MAT1A
Gene ID (NCBI) 4143
RRIDAB_3672946
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00266
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
FC protocol for CL Plus 488 MAT1A antibody CL488-67408Download protocol
Standard Protocols
Click here to view our Standard Protocols