CoraLite® Plus 488-conjugated MAT1A Monoclonal antibody
MAT1A Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F1C1
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67408-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67408 targets MAT1A in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | MAT1A fusion protein Ag3076 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | methionine adenosyltransferase I, alpha |
Synonyms | AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1 |
Calculated Molecular Weight | 395 aa, 44 kDa |
Observed Molecular Weight | 49 kDa |
GenBank Accession Number | BC018359 |
Gene Symbol | MAT1A |
Gene ID (NCBI) | 4143 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q00266 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 MAT1A antibody CL488-67408 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |