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MAVS; VISA Polyclonal antibody

MAVS; VISA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14341-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HuH-7 cells, Jurkat cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAVS Ready-To-Use IHC Kit. MAVS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14341-1-AP targets MAVS; VISA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human
文献引用反应性human, pig, monkey, duck
免疫原 MAVS; VISA fusion protein Ag5655 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial antiviral signaling protein
别名 MAVS, KIAA1271, IPS-1, IPS1, IPS 1
计算分子量 57 kDa
观测分子量 50-55 kDa, 70-75 kDa
GenBank蛋白编号BC044952
基因名称 MAVS
Gene ID (NCBI) 57506
RRIDAB_10548408
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z434
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

实验方案

Product Specific Protocols
WB protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IHC protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IF protocol for MAVS; VISA antibody 14341-1-APDownload protocol
IP protocol for MAVS; VISA antibody 14341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng

Immunity

Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.

Authors - Yujia Wang
WB

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB

Nat Commun

MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.

Authors - Peipei Zhou
humanWB

EMBO J

CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS

Authors - Jia Yi
  • KD Validated
pigWB

Autophagy

Induction of autophagy and suppression of type I IFN secretion by CSFV.

Authors - Baoming Xie
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