验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-14341 targets MAVS; VISA in IF/ICC applications and shows reactivity with human samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | MAVS; VISA fusion protein Ag5655 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | mitochondrial antiviral signaling protein |
别名 | MAVS, KIAA1271, IPS-1, IPS1, IPS 1 |
计算分子量 | 57 kDa |
观测分子量 | 50-55 kDa, 70-75 kDa |
GenBank蛋白编号 | BC044952 |
基因名称 | MAVS |
Gene ID (NCBI) | 57506 |
RRID | AB_2883082 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q7Z434 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 MAVS; VISA antibody CL488-14341 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |