CoraLite®594-conjugated MAdCAM1 Monoclonal antibody
MAdCAM1 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2A12E8
验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy MAdCAM1 Ready-To-Use IHC Kit. MAdCAM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66594-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66594 targets MAdCAM1 in IF-P applications and shows reactivity with Human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human |
| Immunogen | MAdCAM1 fusion protein Ag16184 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Synonyms | hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1 |
| Calculated Molecular Weight | 406 aa, 43 kDa |
| GenBank Accession Number | BC142629 |
| Gene Symbol | MADCAM1 |
| Gene ID (NCBI) | 8174 |
| RRID | AB_2920014 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13477 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MAdCAM1 antibody CL594-66594 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |