IHCeasy MAdCAM1 Ready-To-Use IHC Kit
MAdCAM1 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC0025
Synonyms
hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1
Validation Data Gallery
Product Information
KHC0025 is a ready-to-use IHC kit for staining of MAdCAM1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy MAdCAM1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium. The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1 |