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验证数据展示

  • WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

    WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBIP antibody included
  • IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBIP antibody included
  • IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MBIP Monoclonal antibody, PBS Only

MBIP Monoclonal Antibody for WB, IP, IHC, Indirect ELISA
Cat No. 66102-1-PBS

产品说明书

CloneNo. 2E6H11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IP, IHC, Indirect ELISA

别名

MBIP, MUK binding inhibitory protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.

产品信息

66102-1-PBS targets MBIP in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 MBIP fusion protein Ag18090 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MAP3K12 binding inhibitory protein 1
别名 MBIP, MUK binding inhibitory protein
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC005197
基因名称 MBIP
Gene ID (NCBI) 51562
RRIDAB_2881501
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS73
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.