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  • Featured Product
  • KD/KO Validated

MBIP Monoclonal antibody, PBS Only

MBIP Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6H11

Cat no : 66102-1-PBS

Print datasheet

Synonyms

MBIP, MUK binding inhibitory protein

验证数据展示

  • WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

    WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBIP antibody included
  • IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBIP antibody included
  • IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

    IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.

产品信息

66102-1-PBS targets MBIP in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen MBIP fusion protein Ag18090 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MAP3K12 binding inhibitory protein 1
Synonyms MBIP, MUK binding inhibitory protein
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC005197
Gene Symbol MBIP
Gene ID (NCBI) 51562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS73
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.