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验证数据展示

  • WB analysis using 66102-1-Ig

    WB analysis using 66102-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66102-1-Ig

    WB analysis of HEK-293 using 66102-1-Ig

    WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

  • IHC staining of human kidney using 66102-1-Ig

    IHC staining of human kidney using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MBIP antibody included
  • IHC staining of human kidney using 66102-1-Ig

    IHC staining of human kidney using 66102-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MBIP antibody included
  • IP experiment of HEK-293 using 66102-1-Ig

    IP experiment of HEK-293 using 66102-1-Ig

    IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug.

  • Featured Product
  • KD/KO验证

MBIP Monoclonal antibody

MBIP Monoclonal Antibody for WB, IP, IHC, ELISA
Cat No. 66102-1-Ig

产品说明书

CloneNo. 2E6H11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

MBIP, MUK binding inhibitory protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, human heart tissue, HepG2 cells, LNCaP cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66102-1-Ig targets MBIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MBIP fusion protein Ag18090 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MAP3K12 binding inhibitory protein 1
别名 MBIP, MUK binding inhibitory protein
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC005197
基因名称 MBIP
Gene ID (NCBI) 51562
RRIDAB_2881501
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

实验方案

Product Specific Protocols
WB protocol for MBIP antibody 66102-1-IgDownload protocol
IHC protocol for MBIP antibody 66102-1-IgDownload protocol
IP protocol for MBIP antibody 66102-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols