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验证数据展示

  • FC experiment of HeLa using CL488-66102

    FC experiment of HeLa using CL488-66102

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MBIP (CL488-66102, Clone:2E6H11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MBIP Monoclonal antibody

MBIP Monoclonal Antibody for FC (Intra)
Cat No. CL488-66102

产品说明书

CloneNo. 2E6H11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

FC (Intra)

别名

MUK-binding inhibitory protein, MUK binding inhibitory protein, MAPK upstream kinase-binding inhibitory protein, MAP3K12-binding inhibitory protein 1, BM-015

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MBIP Ready-To-Use IHC Kit. MBIP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66102-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66102 targets MBIP in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 MBIP fusion protein Ag18090 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MAP3K12 binding inhibitory protein 1
别名 MUK-binding inhibitory protein, MUK binding inhibitory protein, MAPK upstream kinase-binding inhibitory protein, MAP3K12-binding inhibitory protein 1, BM-015
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC005197
基因名称 MBIP
Gene ID (NCBI) 51562
RRIDAB_2934447
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS73
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MBIP antibody CL488-66102Download protocol
Standard Protocols
Click here to view our Standard Protocols