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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66837-1-Ig (same clone as 66837-1-PBS)

    WB analysis using 66837-1-Ig (same clone as 66837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBNL1 antibody included
  • IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MBNL1 antibody included
  • IF Staining of HepG2 using 66837-1-Ig (same clone as 66837-1-PBS)

    IF Staining of HepG2 using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66837-1-Ig (MBNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

MBNL1 Monoclonal antibody, PBS Only

MBNL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66837-1-PBS
CloneNo. 1H2C12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MBNL1 Ready-To-Use IHC Kit. MBNL1 primary antibody included.

产品信息

66837-1-PBS targets MBNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen MBNL1 fusion protein Ag7752 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name muscleblind-like (Drosophila)
Synonyms DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC050535
Gene Symbol MBNL1
Gene ID (NCBI) 4154
RRIDAB_2882180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NR56
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.