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验证数据展示

  • IF Staining of A431 using CL488-66837

    IF Staining of A431 using CL488-66837

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MBNL1 antibody (CL488-66837, Clone: 1H2C12 ) at dilution of 1:500.

CoraLite® Plus 488-conjugated MBNL1 Monoclonal antibody

MBNL1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66837

产品说明书

CloneNo. 1H2C12

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

IF/ICC

别名

DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MBNL1 Ready-To-Use IHC Kit. MBNL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66837-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66837 targets MBNL1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 MBNL1 fusion protein Ag7752 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 muscleblind-like (Drosophila)
别名 DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1
计算分子量 42 kDa
观测分子量 40 kDa
GenBank蛋白编号BC050535
基因名称 MBNL1
Gene ID (NCBI) 4154
RRIDAB_2934499
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR56
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MBNL1 antibody CL488-66837Download protocol
Standard Protocols
Click here to view our Standard Protocols