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  • Featured Product
  • KD/KO Validated

MCM2 Monoclonal antibody, PBS Only

MCM2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4D10

Cat no : 66204-1-PBS

Print datasheet

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

验证数据展示

  • WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

    WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

    WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

  • WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

    WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

    IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM2 antibody included
  • IF Staining of MCF-7 using 66204-1-Ig (same clone as 66204-1-PBS)

    IF Staining of MCF-7 using 66204-1-Ig (same clone as 66204-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

产品信息

66204-1-PBS targets MCM2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C