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  • Featured Product
  • KD/KO Validated

MCM2 Monoclonal antibody, PBS Only

MCM2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1F4D10

Cat No : 66204-1-PBS

Print datasheet

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

验证数据展示

  • WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

    WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

    WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

  • WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

    WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

    IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM2 antibody included
  • IF Staining of MCF-7 using 66204-1-Ig (same clone as 66204-1-PBS)

    IF Staining of MCF-7 using 66204-1-Ig (same clone as 66204-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

产品信息

66204-1-PBS targets MCM2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.