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MCM2 Monoclonal antibody

MCM2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4D10

Cat no : 66204-1-Ig

Print datasheet

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

验证数据展示

  • WB analysis of HEK-293 using 66204-1-Ig

    WB analysis of HEK-293 using 66204-1-Ig

    WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

  • WB analysis using 66204-1-Ig

    WB analysis using 66204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66204-1-Ig

    IHC staining of human prostate cancer using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM2 antibody included
  • IF Staining of MCF-7 using 66204-1-Ig

    IF Staining of MCF-7 using 66204-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, A431 cells, 4T1 cells, A549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66204-1-Ig targets MCM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
RRIDAB_2881595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MCM2 antibody 66204-1-IgDownload protocol
IHC protocol for MCM2 antibody 66204-1-IgDownload protocol
IF protocol for MCM2 antibody 66204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols