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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MCM2 Monoclonal antibody

MCM2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F4D10

Cat no : CL488-66204

Print datasheet

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

验证数据展示

  • IF Staining of MCF-7 using CL488-66204

    IF Staining of MCF-7 using CL488-66204

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 MCM2 antibody (CL488-66204, Clone: 1F4D10 ) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66204-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66204 targets MCM2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49736
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM2 antibody CL488-66204Download protocol
Standard Protocols
Click here to view our Standard Protocols