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验证数据展示

  • WB analysis using 84511-5-RR (same clone as 84511-5-PBS)

    WB analysis using 84511-5-RR (same clone as 84511-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-5-RR (MCM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84511-5-RR (same clone as 84511-5-PBS)

    IF Staining of MCF-7 using 84511-5-RR (same clone as 84511-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

    IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM2 antibody included
  • Affinity and Kinetic Characterization of 84511-5-RR

    Affinity and Kinetic Characterization of 84511-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-5-RR against Human MCM2 were performed. The affinity constant is 2.04 nM.

MCM2 Recombinant antibody, PBS Only

MCM2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84511-5-PBS

产品说明书

CloneNo. 241968F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

产品信息

84511-5-PBS targets MCM2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MCM2 fusion protein Ag0798 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 minichromosome maintenance complex component 2
别名 KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28
计算分子量 102 kDa
观测分子量 116-125 kDa
GenBank蛋白编号BC007670
基因名称 MCM2
Gene ID (NCBI) 4171
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP49736
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.