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验证数据展示

  • WB analysis using 84511-5-RR

    WB analysis using 84511-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-5-RR (MCM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 84511-5-RR

    IF Staining of MCF-7 using 84511-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse colon using 84511-5-RR

    IHC staining of mouse colon using 84511-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM2 antibody included
  • Affinity and Kinetic Characterization of 84511-5-RR

    Affinity and Kinetic Characterization of 84511-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-5-RR against Human MCM2 were performed. The affinity constant is 2.04 nM.

MCM2 Recombinant antibody

MCM2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84511-5-RR
CloneNo. 241968F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, A431 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse colon tissue, human cervical squamous cancer tissue, human colon cancer tissue, human placenta tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84511-5-RR targets MCM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 116-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

实验方案

Product Specific Protocols
WB protocol for MCM2 antibody 84511-5-RRDownload protocol
IHC protocol for MCM2 antibody 84511-5-RRDownload protocol
IF protocol for MCM2 antibody 84511-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols