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MCM3 Recombinant antibody

MCM3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242103B9

Cat no : 84792-5-RR

Print datasheet

Synonyms

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

验证数据展示

  • WB analysis using 84792-5-RR

    WB analysis using 84792-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-5-RR (MCM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84792-5-RR

    IHC staining of human colon cancer using 84792-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM3 antibody included
  • FC experiment of HeLa using 84792-5-RR

    FC experiment of HeLa using 84792-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84792-5-RR

    Affinity and Kinetic Characterization of 84792-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84792-5-RR against Human MCM3 were performed. The affinity constant is 73.2 pM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Raji cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MCM3 Ready-To-Use IHC Kit. MCM3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84792-5-RR targets MCM3 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MCM3 fusion protein Ag7966 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name minichromosome maintenance complex component 3
Synonyms P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 102-105 kDa
GenBank Accession NumberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25205
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

实验方案

Product Specific Protocols
WB protocol for MCM3 antibody 84792-5-RRDownload protocol
IHC protocol for MCM3 antibody 84792-5-RRDownload protocol
FC protocol for MCM3 antibody 84792-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols