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Anti-Human MCP-1 Rabbit Recombinant Antibody

MCP-1 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230270A7

Cat No : 98044-1-RR

Print datasheet

Synonyms

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

验证数据展示

  • FC experiment of THP-1 using 98044-1-RR

    FC experiment of THP-1 using 98044-1-RR

    1x10^6 untreated or PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.5 ug Anti-Human MCP-1 Rabbit Recombinant Antibody (98044-1-RR, Clone: 230270A7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inPMA, LPS and Brefeldin A treated THP-1 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98044-1-RR is MCP-1 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC009716
Gene Symbol CCL2
Gene ID (NCBI) 6347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13500
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

实验方案

Product Specific Protocols
FC protocol for MCP-1 antibody 98044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols