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M-CSF Recombinant antibody

M-CSF Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241743C5

Cat no : 84481-4-RR

Print datasheet

Synonyms

M CSF, Lanimostim, CSF-1, CSF1, CSF 1

验证数据展示

  • WB analysis using 84481-4-RR

    WB analysis using 84481-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84481-4-RR (MCSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84481-4-RR

    Affinity and Kinetic Characterization of 84481-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84481-4-RR against Human M-CSF were performed. The affinity constant is 11.4 nM.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, THP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84481-4-RR targets M-CSF in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 1 (macrophage)
Synonyms M CSF, Lanimostim, CSF-1, CSF1, CSF 1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC021117
Gene Symbol M-CSF
Gene ID (NCBI) 1435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

实验方案

Product Specific Protocols
WB protocol for M-CSF antibody 84481-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols