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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MCT1 Polyclonal antibody

MCT1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20139

Print datasheet

Synonyms

FLJ36745, HHF7, MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1

验证数据展示

  • IF Staining of HeLa using CL488-20139

    IF Staining of HeLa using CL488-20139

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 MCT1 antibody (CL488-20139) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SLC16A1/MCT1 Ready-To-Use IHC Kit. SLC16A1/MCT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20139-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20139 targets MCT1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen MCT1 fusion protein Ag14098 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
Synonyms FLJ36745, HHF7, MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1
Calculated Molecular Weight 500 aa, 54 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC026317
Gene Symbol MCT1
Gene ID (NCBI) 6566
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53985
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCT1 antibody CL488-20139Download protocol
Standard Protocols
Click here to view our Standard Protocols