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验证数据展示

  • WB analysis using 84797-4-RR

    WB analysis using 84797-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84797-4-RR (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 84797-4-RR

    IF Staining of mouse skeletal muscle using 84797-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84797-4-RR

    Affinity and Kinetic Characterization of 84797-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84797-4-RR against Human MCT4 were performed. The affinity constant is 3.02 nM.

MCT4 Recombinant antibody

MCT4 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84797-4-RR

产品说明书

CloneNo. 242296E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, ELISA

别名

SLC16A3, MCT-4, MCT3, MCT 4, 242296E3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MCT4 Ready-To-Use IHC Kit. MCT4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84797-4-RR targets MCT4 in WB, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human
免疫原 MCT4 fusion protein Ag18788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
别名 SLC16A3, MCT-4, MCT3, MCT 4, 242296E3
计算分子量 465 aa, 49 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC112269
基因名称 MCT4
Gene ID (NCBI) 9123
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15427
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

实验方案

Product Specific Protocols
WB protocol for MCT4 antibody 84797-4-RRDownload protocol
IF protocol for MCT4 antibody 84797-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols