验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84797-4-RR targets MCT4 in WB, IF-P, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF-P, ELISA Application Description |
Tested Reactivity | human |
Immunogen | MCT4 fusion protein Ag18788 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | solute carrier family 16, member 3 (monocarboxylic acid transporter 4) |
Synonyms | SLC16A3, MCT-4, MCT3, MCT 4, 242296E3 |
Calculated Molecular Weight | 465 aa, 49 kDa |
Observed Molecular Weight | 38-42 kDa |
GenBank Accession Number | BC112269 |
Gene Symbol | MCT4 |
Gene ID (NCBI) | 9123 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | O15427 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.
实验方案
Product Specific Protocols | |
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WB protocol for MCT4 antibody 84797-4-RR | Download protocol |
IF protocol for MCT4 antibody 84797-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |