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验证数据展示

  • WB analysis using 24721-1-AP (same clone as 24721-1-PBS)

    WB analysis using 24721-1-AP (same clone as 24721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24721-1-AP (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MDC1 antibody included
  • IF Staining of HEK-293 using 24721-1-AP (same clone as 24721-1-PBS)

    IF Staining of HEK-293 using 24721-1-AP (same clone as 24721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MDC1 antibody (24721-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MDC1 antibody included

MDC1 Polyclonal antibody, PBS Only

MDC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24721-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MDC1 Ready-To-Use IHC Kit. MDC1 primary antibody included.

产品信息

24721-1-PBS targets MDC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MDC1 fusion protein Ag20135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator of DNA damage checkpoint 1
别名
计算分子量 2089 aa, 227 kDa
GenBank蛋白编号BC152556
基因名称 MDC1
Gene ID (NCBI) 9656
RRIDAB_2879688
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14676
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.