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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MDH1 Monoclonal antibody

MDH1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F9A2

Cat no : CL488-66505

Print datasheet

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

验证数据展示

  • IF Staining of HepG2 using CL488-66505

    IF Staining of HepG2 using CL488-66505

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MDH1 antibody (CL488-66505, Clone: 1F9A2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66505-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66505 targets MDH1 in IF/ICC applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse
Immunogen MDH1 fusion protein Ag8744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name malate dehydrogenase 1, NAD (soluble)
Synonyms Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 36-37 kDa
GenBank Accession NumberBC001484
Gene Symbol MDH1
Gene ID (NCBI) 4190
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40925
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MDH1 antibody CL488-66505Download protocol
Standard Protocols
Click here to view our Standard Protocols