验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66505-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66505 targets MDH1 in IF/ICC applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | MDH1 fusion protein Ag8744 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | malate dehydrogenase 1, NAD (soluble) |
| 别名 | Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2 |
| 计算分子量 | 334 aa, 36 kDa |
| 观测分子量 | 36-37 kDa |
| GenBank蛋白编号 | BC001484 |
| 基因名称 | MDH1 |
| Gene ID (NCBI) | 4190 |
| RRID | AB_2934468 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P40925 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MDH1 antibody CL488-66505 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |