验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy MDH2 Ready-To-Use IHC Kit. MDH2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68878-1-Ig targets MDH2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MDH2 fusion protein Ag7750 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | malate dehydrogenase 2, NAD (mitochondrial) |
| 别名 | MDH 2, MDH, EC:1.1.1.37, 2A9G11 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC001917 |
| 基因名称 | MDH2 |
| Gene ID (NCBI) | 4191 |
| RRID | AB_3670445 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P40926 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MDH2 antibody 68878-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |