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MDH2 Monoclonal antibody

MDH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A9G11

Cat no : 68878-1-Ig

Print datasheet

Synonyms

MDH 2, MDH, EC:1.1.1.37, 2A9G11

验证数据展示

  • WB analysis using 68878-1-Ig

    WB analysis using 68878-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68878-1-Ig (MDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MDH2 Ready-To-Use IHC Kit. MDH2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68878-1-Ig targets MDH2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MDH2 fusion protein Ag7750 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name malate dehydrogenase 2, NAD (mitochondrial)
Synonyms MDH 2, MDH, EC:1.1.1.37, 2A9G11
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001917
Gene Symbol MDH2
Gene ID (NCBI) 4191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).

实验方案

Product Specific Protocols
WB protocol for MDH2 antibody 68878-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols