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  • Featured Product
  • KD/KO Validated

MDM1 Polyclonal antibody

MDM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17575-1-AP

Print datasheet

Synonyms

Nuclear protein MDM1

验证数据展示

  • WB analysis using 17575-1-AP

    WB analysis using 17575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17575-1-AP

    IHC staining of human brain using 17575-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17575-1-AP

    IHC staining of human brain using 17575-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of mouse eye using 17575-1-AP

    IF Staining of mouse eye using 17575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

  • IF Staining of mouse eye using 17575-1-AP

    IF Staining of mouse eye using 17575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

  • IF Staining of mouse eye using 17575-1-AP

    IF Staining of mouse eye using 17575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 17575-1-AP

    IF Staining of hTERT-RPE1 using 17575-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

经过测试的应用

Positive WB detected inmouse retina tissue, Raji cells, rat retina tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17575-1-AP targets MDM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MDM1 fusion protein Ag10514 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mdm1 nuclear protein homolog (mouse)
Synonyms Nuclear protein MDM1
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC028355
Gene Symbol MDM1
Gene ID (NCBI) 56890
RRIDAB_2281929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

实验方案

Product Specific Protocols
WB protocol for MDM1 antibody 17575-1-APDownload protocol
IHC protocol for MDM1 antibody 17575-1-APDownload protocol
IF protocol for MDM1 antibody 17575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cells

Authors - Youlim Son
  • KO Validated
View Article