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ME2 Monoclonal antibody

ME2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

6E6E2

Cat no : 67457-1-Ig

Print datasheet

Synonyms

Malic enzyme 2, ME2, NAD ME

验证数据展示

  • WB analysis using 67457-1-Ig

    WB analysis using 67457-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67457-1-Ig

    IHC staining of human liver cancer using 67457-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 67457-1-Ig

    WB analysis of HeLa using 67457-1-Ig

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, human peripheral blood platelets, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67457-1-Ig targets ME2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen ME2 fusion protein Ag20741 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms Malic enzyme 2, ME2, NAD ME
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 54 kDa, 65 kDa
GenBank Accession NumberBC000147
Gene Symbol ME2
Gene ID (NCBI) 4200
RRIDAB_2882689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23368
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ME2 antibody 67457-1-IgDownload protocol
IHC protocol for ME2 antibody 67457-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nat Commun

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis

Authors - Taiqi Chen
View Article