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ME2 Monoclonal antibody

ME2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67457-1-Ig

产品说明书

CloneNo. 6E6E2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat and More (2)

应用

WB, IHC, ELISA and More (1)

Malic enzyme 2, ME2, NAD ME

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, human peripheral blood platelets, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67457-1-Ig targets ME2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF, IHC
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse
免疫原 ME2 fusion protein Ag20741 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 malic enzyme 2, NAD(+)-dependent, mitochondrial
别名 Malic enzyme 2, ME2, NAD ME
计算分子量 65 kDa
观测分子量 54 kDa, 65 kDa
GenBank蛋白编号BC000147
基因名称 ME2
Gene ID (NCBI) 4200
RRIDAB_2882689
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23368
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ME2 antibody 67457-1-IgDownload protocol
IHC protocol for ME2 antibody 67457-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nat Commun

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis

Authors - Taiqi Chen
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