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  • Featured Product
  • KD/KO Validated

ME2 Monoclonal antibody, PBS Only

ME2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E6E2

Cat No : 67457-1-PBS

Print datasheet

Synonyms

Malic enzyme 2, ME2, NAD ME

验证数据展示

  • WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

产品信息

67457-1-PBS targets ME2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ME2 fusion protein Ag20741 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms Malic enzyme 2, ME2, NAD ME
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 54 kDa, 65 kDa
GenBank Accession NumberBC000147
Gene Symbol ME2
Gene ID (NCBI) 4200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23368
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.