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验证数据展示

  • WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

    WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ME2 Monoclonal antibody, PBS Only

ME2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67457-1-PBS

产品说明书

CloneNo. 6E6E2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

Malic enzyme 2, ME2, NAD ME

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67457-1-PBS targets ME2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ME2 fusion protein Ag20741 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 malic enzyme 2, NAD(+)-dependent, mitochondrial
别名 Malic enzyme 2, ME2, NAD ME
计算分子量 65 kDa
观测分子量 54 kDa, 65 kDa
GenBank蛋白编号BC000147
基因名称 ME2
Gene ID (NCBI) 4200
RRIDAB_2882689
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23368
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.