• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using 83540-1-RR

    IF Staining of HepG2 using 83540-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED12L antibody (83540-1-RR, Clone: 240101C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 83540-1-RR

    IHC staining of mouse brain using 83540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83540-1-RR (MED12L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U2OS using 83540-1-RR

    FC experiment of U2OS using 83540-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug MED12L Recombinant antibody (83540-1-RR, Clone:240101C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MED12L Recombinant antibody

MED12L Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83540-1-RR

产品说明书

CloneNo. 240101C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, KIAA1635

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83540-1-RR targets MED12L in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 MED12L fusion protein Ag34636 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 12-like
别名 Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, KIAA1635
计算分子量240 kDa
GenBank蛋白编号NM_053002
基因名称 MED12L
Gene ID (NCBI) 116931
RRIDAB_3671161
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86YW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody can recognize all the isoform of MED12L. This antibody has no cross-reaction to MED12.

实验方案

Product Specific Protocols
IHC protocol for MED12L antibody 83540-1-RRDownload protocol
IF protocol for MED12L antibody 83540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols