CoraLite® Plus 488-conjugated MED12L Recombinant antibody
MED12L Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240101C11
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 83540-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83540 targets MED12L in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | MED12L fusion protein Ag34636 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | mediator complex subunit 12-like |
| Synonyms | Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, KIAA1635 |
| Calculated Molecular Weight | 240 kDa |
| GenBank Accession Number | NM_053002 |
| Gene Symbol | MED12L |
| Gene ID (NCBI) | 116931 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86YW9 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody can recognize all the isoform of MED12L. This antibody has no cross-reaction to MED12.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MED12L antibody CL488-83540 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |