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验证数据展示

  • WB analysis of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    WB analysis of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11566-1-AP (same clone as 11566-1-PBS)

    IHC staining of human prostate cancer using 11566-1-AP (same clone as 11566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    IP experiment of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

    IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 11566-1-AP (same clone as 11566-1-PBS)

    IF Staining of SH-SY5Y using 11566-1-AP (same clone as 11566-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MED15 Polyclonal antibody, PBS Only

MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11566-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11566-1-PBS targets MED15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 MED15 fusion protein Ag2143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mediator complex subunit 15
别名
计算分子量 788 aa, 87 kDa
观测分子量 105 kDa
GenBank蛋白编号BC017110
基因名称 MED15
Gene ID (NCBI) 51586
RRIDAB_2141975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RN5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).