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  • Featured Product
  • KD/KO Validated

MED30 Monoclonal antibody

MED30 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D7C4

Cat no : 67038-1-Ig

Print datasheet

Synonyms

MED30, mediator complex subunit 30, THRAP6, TRAP25

验证数据展示

  • WB analysis using 67038-1-Ig

    WB analysis using 67038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67038-1-Ig (MED30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67038-1-Ig

    WB analysis of HEK-293 using 67038-1-Ig

    WB result of MED30 antibody (67038-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, pig brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67038-1-Ig targets MED30 in WB, ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, rat, pig
Immunogen MED30 fusion protein Ag10235 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mediator complex subunit 30
Synonyms MED30, mediator complex subunit 30, THRAP6, TRAP25
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC008226
Gene Symbol MED30
Gene ID (NCBI) 90390
RRIDAB_2882352
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

实验方案

Product Specific Protocols
WB protocol for MED30 antibody 67038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols