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验证数据展示

  • WB analysis using 67038-1-Ig

    WB analysis using 67038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67038-1-Ig (MED30 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67038-1-Ig

    WB analysis of HEK-293 using 67038-1-Ig

    WB result of MED30 antibody (67038-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

MED30 Monoclonal antibody

MED30 Monoclonal Antibody for WB, ELISA
Cat No. 67038-1-Ig

产品说明书

CloneNo. 1D7C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig

应用

WB, ELISA

别名

MED30, mediator complex subunit 30, THRAP6, TRAP25

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, pig brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67038-1-Ig targets MED30 in WB, ELISA applications and shows reactivity with Human, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, rat, pig
免疫原 MED30 fusion protein Ag10235 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 30
别名 MED30, mediator complex subunit 30, THRAP6, TRAP25
计算分子量 178 aa, 20 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008226
基因名称 MED30
Gene ID (NCBI) 90390
RRIDAB_2882352
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96HR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

实验方案

Product Specific Protocols
WB protocol for MED30 antibody 67038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols