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验证数据展示

  • FC experiment of HeLa using CL488-67038

    FC experiment of HeLa using CL488-67038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED30 (CL488-67038, Clone:1D7C4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MED30 Monoclonal antibody

MED30 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67038

产品说明书

CloneNo. 1D7C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig

应用

FC (Intra)

别名

Thyroid hormone receptor-associated protein complex 25 kDa component, Thyroid hormone receptor-associated protein 6, THRAP6, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67038-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67038 targets MED30 in FC (Intra) applications and shows reactivity with Human, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat, pig
免疫原 MED30 fusion protein Ag10235 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mediator complex subunit 30
别名 Thyroid hormone receptor-associated protein complex 25 kDa component, Thyroid hormone receptor-associated protein 6, THRAP6, Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30
计算分子量 178 aa, 20 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008226
基因名称 MED30
Gene ID (NCBI) 90390
RRIDAB_2934515
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96HR3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MED30 antibody CL488-67038Download protocol
Standard Protocols
Click here to view our Standard Protocols