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验证数据展示

  • WB analysis using 84184-4-RR (same clone as 84184-4-PBS)

    WB analysis using 84184-4-RR (same clone as 84184-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3, Human MED6 Recombinant Matched Antibody Pair - PBS only. 84184-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7541. 84184-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

  • Sample test of MP01087-3

    Sample test of MP01087-3

    The mean MED6 concentration was determined to be 7.45 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 16.00 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 13.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load.

  • FC experiment of A431 using 84184-4-RR (same clone as 84184-4-PBS)

    FC experiment of A431 using 84184-4-RR (same clone as 84184-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3, MED6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84184-4-PBS. Detection antibody: 84184-2-PBS. Standard: Ag7541. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody, PBS Only (Capture)

MED6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84184-4-PBS

产品说明书

CloneNo. 241382H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84184-4-PBS targets MED6 as part of a matched antibody pair:

MP01087-3: 84184-4-PBS capture and 84184-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 MED6 fusion protein Ag7541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 6
别名 NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component
计算分子量 28 kDa
观测分子量33 kDa
GenBank蛋白编号BC004106
基因名称 MED6
Gene ID (NCBI) 10001
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75586
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.