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验证数据展示

  • WB analysis using 84184-4-RR

    WB analysis using 84184-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84184-4-RR

    FC experiment of A431 using 84184-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody

MED6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84184-4-RR

产品说明书

CloneNo. 241382H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, mouse colon tissue, mouse brain tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84184-4-RR targets MED6 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 MED6 fusion protein Ag7541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 6
别名 NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component
计算分子量 28 kDa
观测分子量33 kDa
GenBank蛋白编号BC004106
基因名称 MED6
Gene ID (NCBI) 10001
RRIDAB_3671738
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED6 antibody 84184-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols