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MED6 Recombinant antibody

MED6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241382H2

Cat no : 84184-4-RR

Print datasheet

Synonyms

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

验证数据展示

  • WB analysis using 84184-4-RR

    WB analysis using 84184-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84184-4-RR

    FC experiment of A431 using 84184-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, mouse colon tissue, mouse brain tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84184-4-RR is MED6 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen MED6 fusion protein Ag7541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mediator complex subunit 6
Synonyms NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component
Calculated Molecular Weight 28 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

实验方案

Product Specific Protocols
WB protocol for MED6 antibody 84184-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols